An improved Fourier-Transform Infrared Spectroscopy combined with partial least squares regression for rapid quantification of total aflatoxins in commercial chicken feeds and food grains.

ABSTRACT:

Objective

This study aims to develop and validate an Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR) spectroscopic technique combined with a partial least squares regression (PLSR) model for rapid quantification and monitoring of aflatoxins in chicken feeds and food grains.

Materials and methods

A model of ATR-FTIR-PLSR was developed using ATR-FTIR spectra of mixed aflatoxin standards in 100% acetonitrile (112 samples) and 75% methanol (112 samples), validated by testing its prediction on 125 feed/food samples spiked with variable concentrations of aflatoxins, and applied to screen 660 samples of commercial chicken feeds and food grains from Nigerian and Malaysian markets for total aflatoxins, for which the dietary exposure risks to aflatoxins

SUBMITTER: Salisu B 

PROVIDER: S-EPMC9597927 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature