Highly Sensitive Determination of Antibiotic Residues in Aquatic Products by High-Performance Liquid Chromatography-Tandem Mass Spectrometry.

ABSTRACT: Antibiotic drug residues are crucial to ensure food safety and minimize risk to human health. Herein, a sensitive high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed and validated for the determination of antibiotic residues (mainly amphenicols) consisting of chloramphenicol (CAP), thiamphenicol (TAP), florfenicol (FF), and florfenicol amine (FFA) in aquatic products. Amphenicols were well separated on a Kinetex F5 (100 mm × 3.0 mm, 2.6 µm) chromatographic column with the mobile phases of 1 mM ammonium acetate aqueous solution and methanol solution and measured after positive and negative electrospray ionizations using four internal standards. To our knowledge, it was the first time to report the good performance of F5 column and four internal

SUBMITTER: Ye H 

PROVIDER: S-EPMC9598229 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature