Nested Real-Time PCR Assessment of Vertical Transmission of Sandalwood Spike Phytoplasma ('Ca. Phytoplasma asteris').

ABSTRACT: The Sandalwood Spike disease (SSD)-related to 'Ca. Phytoplasma asteris' has threatened the existence of sandalwood in India. The epidemiology of SSD is still poorly understood despite the efforts to understand the involvement of insect vectors in SSD transmission and alternate plant hosts over the last two decades. Apart from the transmission of SSD phytoplasma through insect vectors, the information on vertical transmission is entirely unknown. Over 200 seeds from SSD-affected trees and over 500 seedlings generated using commercially purchased seeds were screened for the presence of SSD phytoplasma to understand the vertical transmission in an insect-free environment. The end-point nested PCR and real-time nested PCR-based screening revealed an alarming rate of 38.66% and 23.23% ph

SUBMITTER: Kirdat K 

PROVIDER: S-EPMC9598993 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature