Deciphering the Alphabet of Disorder-Glu and Asp Act Differently on Local but Not Global Properties.

ABSTRACT: Compared to folded proteins, the sequences of intrinsically disordered proteins (IDPs) are enriched in polar and charged amino acids. Glutamate is one of the most enriched amino acids in IDPs, while the chemically similar amino acid aspartate is less enriched. So far, the underlying functional differences between glutamates and aspartates in IDPs remain poorly understood. In this study, we examine the differential effects of aspartate and glutamates in IDPs by comparing the function and conformational ensemble of glutamate and aspartate variants of the disordered protein Dss1, using a range of assays, including interaction studies, nuclear magnetic resonance spectroscopy, small-angle X-ray scattering and molecular dynamics simulation. First, we analyze the sequences of the rapidly growing

SUBMITTER: Roesgaard MA 

PROVIDER: S-EPMC9599281 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature