Common Patterns of Hydrolysis Initiation in P-loop Fold Nucleoside Triphosphatases.

ABSTRACT: The P-loop fold nucleoside triphosphate (NTP) hydrolases (also known as Walker NTPases) function as ATPases, GTPases, and ATP synthases, are often of medical importance, and represent one of the largest and evolutionarily oldest families of enzymes. There is still no consensus on their catalytic mechanism. To clarify this, we performed the first comparative structural analysis of more than 3100 structures of P-loop NTPases that contain bound substrate Mg-NTPs or their analogues. We proceeded on the assumption that structural features common to these P-loop NTPases may be essential for catalysis. Our results are presented in two articles. Here, in the first, we consider the structural elements that stimulate hydrolysis. Upon interaction of P-loop NTPases with their cognate activating partne

SUBMITTER: Kozlova MI 

PROVIDER: S-EPMC9599529 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature