Construction and Analysis of an Enzyme-Constrained Metabolic Model of Corynebacterium glutamicum.

ABSTRACT: The genome-scale metabolic model (GEM) is a powerful tool for interpreting and predicting cellular phenotypes under various environmental and genetic perturbations. However, GEM only considers stoichiometric constraints, and the simulated growth and product yield values will show a monotonic linear increase with increasing substrate uptake rate, which deviates from the experimentally measured values. Recently, the integration of enzymatic constraints into stoichiometry-based GEMs was proven to be effective in making novel discoveries and predicting new engineering targets. Here, we present the first genome-scale enzyme-constrained model (ecCGL1) for Corynebacterium glutamicum reconstructed by integrating enzyme kinetic data from various sources using a ECMpy workflow based on the hi

SUBMITTER: Niu J 

PROVIDER: S-EPMC9599660 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature