Colorimetric Detection of Urease-Producing Microbes Using an Ammonia-Responsive Flexible Film Sensor.

ABSTRACT: Urease-producing (ureolytic) microbes have given rise to environmental and public health concerns because they are thought to contribute to emissions of ammonia and to be a virulence factor for infections. Therefore, it is highly important to have the ability to detect such microbes. In this study, a poly(dimethylsiloxane) (PDMS)-based colorimetric film sensor was employed for the detection of urease-producing microbes. The sensor was able to detect the enzyme activity of commercially available urease, as the color and absorbance spectrum of the sensor was observed to change upon being exposed to the reaction catalyzed by urease. The ratio of the absorbance of the sensor at 640 nm to that at 460 nm (A₆₄₀/A₄₆₀) was linearly proportional to the amount of urease present.

SUBMITTER: Chang Y 

PROVIDER: S-EPMC9599750 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature