Quantifying Coexistence Concentrations in Multi-Component Phase-Separating Systems Using Analytical HPLC.

ABSTRACT: Over the last decade, evidence has accumulated to suggest that numerous instances of cellular compartmentalization can be explained by the phenomenon of phase separation. This is a process by which a macromolecular solution separates spontaneously into dense and dilute coexisting phases. Semi-quantitative, in vitro approaches for measuring phase boundaries have proven very useful in determining some key features of biomolecular condensates, but these methods often lack the precision necessary for generating quantitative models. Therefore, there is a clear need for techniques that allow quantitation of coexisting dilute and dense phase concentrations of phase-separating biomolecules, especially in systems with more than one type of macromolecule. Here, we report the design and deployment of

SUBMITTER: Bremer A 

PROVIDER: S-EPMC9599810 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature