Comparative Analysis of Mesophilic YqfB-Type Amidohydrolases.

ABSTRACT: The widespread superfamily of the human activating signal cointegrator homology (ASCH) domain was identified almost 20 years ago; however, the amount of experimental data regarding the biological function of the domain is scarce. With this study, we aimed to determine the putative cellular functions of four hypothetical ASCH domain-containing amidohydrolase YqfB analogues by investigating their activity towards various N-acylated cytosine derivatives, including potential nucleoside-derived prodrugs, as well as their ability to bind/degrade nucleic acids in vitro. According to determined kinetic parameters, N⁴-acetylcytidine is assumed to be the primary substrate for amidohydrolases. Despite the similarity to the proteins containing the PUA domain, no nucleic acid b

SUBMITTER: Statkeviciute R 

PROVIDER: S-EPMC9599836 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature