Systematic Identification of CpxRA-Regulated Genes and Their Roles in Escherichia coli Stress Response.

ABSTRACT: The two-component system CpxRA can sense environmental stresses and regulate transcription of a wide range of genes for the purpose of adaptation. Despite extensive research on this system, the identification of the CpxR regulon is not systematic or comprehensive. Herein, genome-wide screening was performed using a position-specific scoring matrix, resulting in the discovery of more than 10,000 putative CpxR binding sites, which provides an extensive and selective set of targets based on sequence. More than half of the candidate genes ultimately selected (73/97) were experimentally confirmed to be CpxR-regulated genes through experimental analysis. These genes are involved in various physiological functions, indicating that the CpxRA system regulates complex cellular processes. The study a

SUBMITTER: Zhao Z 

PROVIDER: S-EPMC9600279 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature