Modulation of Neutrophil Activity by Soluble Complement Cleavage Products-An In-Depth Analysis.

ABSTRACT: The cellular and fluid phase-innate immune responses of many diseases predominantly involve activated neutrophil granulocytes and complement factors. However, a comparative systematic analysis of the early impact of key soluble complement cleavage products, including anaphylatoxins, on neutrophil granulocyte function is lacking. Neutrophil activity was monitored by flow cytometry regarding cellular (electro-)physiology, cellular activity, and changes in the surface expression of activation markers. The study revealed no major effects induced by C3a or C4a on neutrophil functions. By contrast, exposure to C5a or C5a des-Arg stimulated neutrophil activity as reflected in changes in membrane potential, intracellular pH, glucose uptake, and cellular size. Similarly, C5a and C5a des-Arg but no

SUBMITTER: Wohlgemuth L 

PROVIDER: S-EPMC9600402 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature