Methylocystis sp. Strain SC2 Acclimatizes to Increasing NH₄⁺ Levels by a Precise Rebalancing of Enzymes and Osmolyte Composition.

ABSTRACT: A high NH₄⁺ load is known to inhibit bacterial methane oxidation. This is due to a competition between CH₄ and NH₃ for the active site of particulate methane monooxygenase (pMMO), which converts CH₄ to CH₃OH. Here, we combined global proteomics with amino acid profiling and nitrogen oxides measurements to elucidate the cellular acclimatization response of Methylocystis sp. strain SC2 to high NH₄⁺ levels. Relative to 1 mM NH₄⁺, a high (50 mM and 75 mM) NH₄⁺ load under CH₄-replete conditions significantly increased the lag phase duration required for proteome adjustment. The number of differentially regulated proteins was highly significantly c

SUBMITTER: Guo K 

PROVIDER: S-EPMC9600857 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature