Top-Down, Knowledge-Based Genetic Reduction of Yeast Central Carbon Metabolism.

ABSTRACT: Saccharomyces cerevisiae, whose evolutionary past includes a whole-genome duplication event, is characterized by a mosaic genome configuration with substantial apparent genetic redundancy. This apparent redundancy raises questions about the evolutionary driving force for genomic fixation of "minor" paralogs and complicates modular and combinatorial metabolic engineering strategies. While isoenzymes might be important in specific environments, they could be dispensable in controlled laboratory or industrial contexts. The present study explores the extent to which the genetic complexity of the central carbon metabolism (CCM) in S. cerevisiae, here defined as the combination of glycolysis, the pentose phosphate pathway, the tricarboxylic acid cycle, and a limited number of related pathways an

SUBMITTER: Postma ED 

PROVIDER: S-EPMC9600970 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature