β-COP Regulates TWIK1/TREK1 Heterodimeric Channel-Mediated Passive Conductance in Astrocytes.

ABSTRACT: Mature astrocytes are characterized by a K⁺ conductance (passive conductance) that changes with a constant slope with voltage, which is involved in K⁺ homeostasis in the brain. Recently, we reported that the tandem of pore domains in a weak inward rectifying K⁺ channel (TWIK1 or KCNK1) and TWIK-related K⁺ channel 1 (TREK1 or KCNK2) form heterodimeric channels that mediate passive conductance in astrocytes. However, little is known about the binding proteins that regulate the function of the TWIK1/TREK1 heterodimeric channels. Here, we found that β-coat protein (COP) regulated the surface expression and activity of the TWIK1/TREK1 heterodimeric channels in astrocytes. β-COP binds directly to TREK1 but not TWIK1 in a heterologous expression system.

SUBMITTER: Kim SS 

PROVIDER: S-EPMC9600989 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature