A method for selective ¹⁹ F-labeling absent of probe sequestration (SLAPS).

ABSTRACT: Fluorine (¹⁹ F) offers several distinct advantages for biomolecular nuclear magnetic resonance spectroscopy such as no background signal, 100% natural abundance, high sensitivity, and a large chemical shift range. Exogenous cysteine-reactive ¹⁹ F-probes have proven especially indispensable for characterizing large, challenging systems that are less amenable to other isotopic labeling strategies such as G protein-coupled receptors. As fluorine linewidths are inherently broad, limiting reactions with offsite cysteines is critical for spectral simplification and accurate deconvolution of component peaks-especially when analyzing systems with intermediate to slow timescale conformational exchange. Here, we uncovered noncovalent probe sequestration by detergent proteomicel

SUBMITTER: Dixon AD 

PROVIDER: S-EPMC9601782 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature