MiR-125-3p and miR-276b-3p Regulate the Spermatogenesis of Bactrocera dorsalis by Targeting the orb2 Gene.

ABSTRACT: Bactrocera dorsalis is considered a major threat to horticultural crops. It has evolved resistance against insecticides. It is believed that development of new methods is highly desirable to control this destructive agricultural pest. Sterile insect technique is emerging as a potential tool to control this insect pest by reducing their reproductive ability. Here we report that orb2 has high expression in the testis of B. dorsalis which is the target of miR-125-3p and miR-276b-3p and plays a critical role in the spermatogenesis. Dual luciferase reporter assay using HEKT293 cells demonstrates that orb2 gene is downregulated by miR-125-3p and miR-276b-3p and is a common target of these miRNAs. Dietary treatment of adult male flies separately and in combination of a

SUBMITTER: Sohail S 

PROVIDER: S-EPMC9601815 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature