The Characterization of a Novel D-allulose 3-Epimerase from Blautia produca and Its Application in D-allulose Production.

ABSTRACT: D-allulose is a natural rare sugar with important physiological properties that is used in food, health care items, and even the pharmaceutical industry. In the current study, a novel D-allulose 3-epimerase gene (Bp-DAE) from the probiotic strain Blautia produca was discovered for the production and characterization of an enzyme known as Bp-DAE that can epimerize D-fructose into D-allulose. Bp-DAE was strictly dependent on metals (Mn²⁺ and Co²⁺), and the addition of 1 mM of Mn²⁺ could enhance the half-life of Bp-DAE at 55 °C from 60 to 180 min. It exhibited optimal activity in a pH of 8 and 55 °C, and the K_m values of Bp-DAE for the different substrates D-fructose and D-allulose were 235.7 and 150.7 mM, respectively. Bp-DAE was used

SUBMITTER: Tang X 

PROVIDER: S-EPMC9601914 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature