Reference Gene Selection for qPCR Analysis in Schima superba under Abiotic Stress.

ABSTRACT: Quantitative real-time PCR (qPCR) is an indispensable technique for gene expression analysis in modern molecular biology. The selection and evaluation of suitable reference genes is a prerequisite for accurate gene expression analysis. Schimasuperba is a valuable tree species that is environmentally adaptable and highly fire-resistant. In this study, 12 candidate reference genes were selected to check their stability of gene expression in different tissues under abiotic stresses: cold stress, salt stress, and drought stress by ΔCt, geNorm, NormFinder, BestKeeper, and RefFinder. The results indicated that AP-2 was the most stably expressed overall and for the cold stress and drought stress. eIF-5α gene expression was the most stable under the salt stress treatmen

SUBMITTER: Yao J 

PROVIDER: S-EPMC9601953 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature