Culturomics of Bacteria from Radon-Saturated Water of the World's Oldest Radium Mine.

ABSTRACT: Balneotherapeutic water springs, such as those with thermal, saline, sulfur, or any other characteristics, have recently been the subject of phylogenetic studies with a closer focus on the description and/or isolation of phylogenetically novel or biotechnologically interesting microorganisms. Generally, however, most such microorganisms are rarely obtained in pure culture or are even, for now, unculturable under laboratory conditions. In this culture-dependent study of radioactive water springs of Jáchymov (Joachimstahl), Czech Republic, we investigated a combination of classical cultivation approaches with those imitating sampling source conditions. Using these environmentally relevant cultivation approaches, over 1,000 pure cultures were successfully isolated from 4 radioactive springs.

SUBMITTER: Kapinusova G 

PROVIDER: S-EPMC9602452 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature