Development and Optimization of a Selective Whole-Genome Amplification To Study Plasmodium ovale Spp.

ABSTRACT: Since 2010, the human-infecting malaria parasite Plasmodium ovale spp. has been divided into two genetically distinct species, P. ovale wallikeri and P. ovale curtisi. In recent years, application of whole-genome sequencing (WGS) to P. ovale spp. allowed to get a better understanding of its evolutionary history and discover some specific genetic patterns. Nevertheless, WGS data from P. ovale spp. are still scarce due to several drawbacks, including a high level of human DNA contamination in blood samples, infections with commonly low parasite density, and the lack of robust in vitro culture. Here, we developed two selective whole-genome amplification (sWGA) protocols that were tested on six P. ovale wallikeri and five P. ovale curtisi mono-infection clini

SUBMITTER: Joste V 

PROVIDER: S-EPMC9602584 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature