Culturomics to Investigate the Endometrial Microbiome: Proof-of-Concept.

ABSTRACT: The microbiome of the reproductive tract has been associated with (sub)fertility and it has been suggested that dysbiosis reduces success rates and pregnancy outcomes. The endometrial microbiome is of particular interest given the potential impact on the embryo implantation. To date, all endometrial microbiome studies have applied a metagenomics approach. A sequencing-based technique, however, has its limitations, more specifically in adequately exploring low-biomass settings, such as intra-uterine/endometrial samples. In this proof-of-concept study, we demonstrate the applicability of culturomics, a high-throughput culturing approach, to investigate the endometrial microbiome. Ten subfertile women undergoing diagnostic hysteroscopy and endometrial biopsy, as part of their routine work-up

SUBMITTER: Vanstokstraeten R 

PROVIDER: S-EPMC9602868 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature