ABSTRACT: This study describes the complete mitochondrial genome sequence of the scyphozoan Acromitus flagellatus (Maas, 1903), a blooming jellyfish found in the coastal areas of Hainan, China. Its mitochondrial DNA is 16,779 bp in length and has a linear structure, comprising 13 protein-coding genes (PCGs), two rRNAs (s-rRNA and l-rRNA), and two tRNAs (trna-W-TCA and trna-M-CAT). A + T content was 65.39% (A: 29.27%, C: 16.59%, G: 18.03%, and T: 36.12%). ATG was the start codon in 11 PCGs: COX1, COX2, ATP8, ATP6, COX3, NAD2, NAD6, NAD4l, NAD1, NAD4, and COB. NAD5 and NAD3 had GTG as the start codon. TAG was the stop codon for COX2, NAD6, and COB. The other 10 PCGs were terminated by