Insights into Muscle Contraction Derived from the Effects of Small-Molecular Actomyosin-Modulating Compounds.

ABSTRACT: Bottom-up mechanokinetic models predict ensemble function of actin and myosin based on parameter values derived from studies using isolated proteins. To be generally useful, e.g., to analyze disease effects, such models must also be able to predict ensemble function when actomyosin interaction kinetics are modified differently from normal. Here, we test this capability for a model recently shown to predict several physiological phenomena along with the effects of the small molecular compound blebbistatin. We demonstrate that this model also qualitatively predicts effects of other well-characterized drugs as well as varied concentrations of MgATP. However, the effects of one compound, amrinone, are not well accounted for quantitatively. We therefore systematically varied key model parameter

SUBMITTER: Mansson A 

PROVIDER: S-EPMC9603234 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature