Phenotypical Flexibility of the EGFRvIII-Positive Glioblastoma Cell Line and the Multidirectional Influence of TGFβ and EGF on These Cells-EGFRvIII Appears as a Weak Oncogene.

ABSTRACT:

Background

The biological role of EGFRvIII (epidermal growth factor receptor variant three) remains unclear.

Methods

Three glioblastoma DK-MG sublines were tested with EGF (epidermal growth factor) and TGFβ (transforming growth factor β). Sublines were characterized by an increased percentage of EGFRvIII-positive cells and doubling time (DK-MG^low to DK-MG^extra-high), number of amplicons, and EGFRvIII mRNA expression. The influence of the growth factors on primary EGFRvIII positive glioblastomas was assessed.

Results

The overexpression of exoEGFRvIII in DK-MG^high did not convert them into DK-MG^extra-high, and this overexpression did not change DK-MG^low to DK-MG^high; however, the overexpression of RAS

SUBMITTER: Wlodarczyk A 

PROVIDER: S-EPMC9603514 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature