The Brain Protein Acylation System Responds to Seizures in the Rat Model of PTZ-Induced Epilepsy.

ABSTRACT: Abnormal energy expenditure during seizures and metabolic regulation through post-translational protein acylation suggest acylation as a therapeutic target in epilepsy. Our goal is to characterize an interplay between the brain acylation system components and their changes after seizures. In a rat model of pentylenetetrazole (PTZ)-induced epilepsy, we quantify 43 acylations in 29 cerebral cortex proteins; levels of NAD⁺; expression of NAD⁺-dependent deacylases (SIRT2, SIRT3, SIRT5); activities of the acyl-CoA-producing/NAD⁺-utilizing complexes of 2-oxoacid dehydrogenases. Compared to the control group, acylations of 14 sites in 11 proteins are found to differ significantly after seizures, with six of the proteins involved in glycolysis and energy metabolism

SUBMITTER: Zavileyskiy LG 

PROVIDER: S-EPMC9603846 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature