Effect of Intragenomic Sequence Heterogeneity among Multiple 16S rRNA Genes on Species Identification of <i>Elizabethkingia</i>.

ABSTRACT: Accurate identification of Elizabethkingia species mostly requires the use of molecular techniques, and 16S rRNA gene sequencing is generally considered the method of choice. In this study, we evaluated the effect of intraspecific diversity among the multiple copies of the 16S rRNA gene on the accuracy of species identification in the genus Elizabethkingia. Sequences of 16S rRNA genes obtained from the 32 complete whole-genome sequences of Elizabethkingia deposited in GenBank and from 218 clinical isolates collected from 5 hospitals in Taiwan were analyzed. Four or five copies of 16S rRNA were identified in the Elizabethkingia species with complete genome sequences. The dissimilarity among the copies of the16S rRNA gene was <1% in all Elizabethkingia stra

SUBMITTER: Lin JN 

PROVIDER: S-EPMC9604143 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature