Computational Modeling and Imaging of the Intracellular Oxygen Gradient.

ABSTRACT: Computational modeling can provide a mechanistic and quantitative framework for describing intracellular spatial heterogeneity of solutes such as oxygen partial pressure (pO₂). This study develops and evaluates a finite-element model of oxygen-consuming mitochondrial bioenergetics using the COMSOL Multiphysics program. The model derives steady-state oxygen (O₂) distributions from Fickian diffusion and Michaelis-Menten consumption kinetics in the mitochondria and cytoplasm. Intrinsic model parameters such as diffusivity and maximum consumption rate were estimated from previously published values for isolated and intact mitochondria. The model was compared with experimental data collected for the intracellular and mitochondrial pO₂ levels in human cervical ca

SUBMITTER: Sedlack AJH 

PROVIDER: S-EPMC9604273 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature