MTOR Contributes to the Proteome Diversity through Transcriptome-Wide Alternative Splicing.

ABSTRACT: The mammalian target of rapamycin (mTOR) pathway is crucial in energy metabolism and cell proliferation. Previously, we reported transcriptome-wide 3'-untranslated region (UTR) shortening by alternative polyadenylation upon mTOR activation and its impact on the proteome. Here, we further interrogated the mTOR-activated transcriptome and found that hyperactivation of mTOR promotes transcriptome-wide exon skipping/exclusion, producing short isoform transcripts from genes. This widespread exon skipping confers multifarious regulations in the mTOR-controlled functional proteomics: AS in coding regions widely affects the protein length and functional domains. They also alter the half-life of proteins and affect the regulatory post-translational modifications. Among the RNA processing factors di

SUBMITTER: Cheng S 

PROVIDER: S-EPMC9604279 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature