Cloning and characterization of the gene encoding the glutamate dehydrogenase of Streptococcus suis serotype 2.

ABSTRACT: Given the lack of effective vaccines to control Streptococcus suis infection and the lack of a rapid and reliable molecular diagnostic assay to detect its infection, a polyclonal antibody was raised against the whole-cell protein of S. suis type 2 and used to screen an S. suis gene library in an effort to identify protective antigen(s) and antigens of diagnostic importance. A clone that produced a 45-kDa S. suis-specific protein was identified by Western blotting. Restriction analysis showed that the gene encoding the 45-kDa protein was present on a 1.6-kb pair DraI region on the cloned chromosomal fragment. The nucleotide sequence contained an open reading frame that encoded a polypeptide of 448 amino acid residues with a calculated molecular mass of 48.8 kDa, in close agreement with the

SUBMITTER: Okwumabua O 

PROVIDER: S-EPMC96045 | biostudies-literature | 2001 Mar

REPOSITORIES: biostudies-literature