Regulator of G Protein Signaling Proteins Control Growth, Development and Cellulase Production in Neurospora crassa.

ABSTRACT: Heterotrimeric (αβγ) G protein signaling pathways are critical environmental sensing systems found in eukaryotic cells. Exchange of GDP for GTP on the Gα subunit leads to its activation. In contrast, GTP hydrolysis on the Gα is accelerated by Regulator of G protein Signaling (RGS) proteins, resulting in a return to the GDP-bound, inactive state. Here, we analyzed growth, development and extracellular cellulase production in strains with knockout mutations in the seven identified RGS genes (rgs-1 to rgs-7) in the filamentous fungus, Neurospora crassa. We compared phenotypes to those of strains with either knockout mutations or expressing predicted constitutively activated, GTPase-deficient alleles for each of the three Gα subunit genes (gna-1^Q204L,

SUBMITTER: Cabrera IE 

PROVIDER: S-EPMC9604755 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature