Procedure for spotted fever group Rickettsia isolation from limited clinical blood specimens.

ABSTRACT:

Background

Current isolation techniques for spotted fever group Rickettsia from clinical samples are laborious and are limited to tissue, blood and blood derivatives with volumes ideally greater than 1 mL. We validated the use of simplified methodologies for spotted fever group Rickettsia culture isolation that overcome sample volume limitations and provide utility in clinical diagnostics and research studies.

Methodology/principal findings

A modified cell culture method is evaluated for the isolation of Rickettsia ssp. from human diagnostic samples. Culture sampling method, culture platform, and growth phase analysis were evaluated to determine best practices for optimal culture isolation conditions. Rickettsial isolates (R. conorii, R. rickettsii, and R. parkeri) were grow

SUBMITTER: Condit ME 

PROVIDER: S-EPMC9605293 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature