Novel fast pathogen diagnosis method for severe pneumonia patients in the intensive care unit: randomized clinical trial.

ABSTRACT:

Background

Severe pneumonia is one of the common acute diseases caused by pathogenic microorganism infection, especially by pathogenic bacteria, leading to sepsis with a high morbidity and mortality rate. However, the existing bacteria cultivation method cannot satisfy current clinical needs requiring rapid identification of bacteria strain for antibiotic selection. Therefore, developing a sensitive liquid biopsy system demonstrates the enormous value of detecting pathogenic bacterium species in pneumonia patients.

Methods

In this study, we developed a tool named Species-Specific Bacterial Detector (SSBD, pronounce as 'speed') for detecting selected bacterium. Newly designed diagnostic tools combining specific DNA-tag screened by our algorithm and CRISPR/Cas12a, which were f

SUBMITTER: Wang Y 

PROVIDER: S-EPMC9605691 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature