Engineered fast-dissociating antibody fragments for multiplexed super-resolution microscopy.

ABSTRACT: Image reconstruction by integrating exchangeable single-molecule localization (IRIS) achieves multiplexed super-resolution imaging by high-density labeling with fast exchangeable fluorescent probes. However, previous methods to develop probes for individual targets required a great amount of time and effort. Here, we introduce a method for generating recombinant IRIS probes with a new mutagenesis strategy that can be widely applied to existing antibody sequences. Several conserved tyrosine residues at the base of complementarity-determining regions were identified as candidate sites for site-directed mutagenesis. With a high probability, mutations at candidate sites accelerated the off rate of recombinant antibody-based probes without compromising specific binding. We were able to develop

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC9606137 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature