Crystal structure of the Na⁺/H⁺ antiporter NhaA at active pH reveals the mechanistic basis for pH sensing.

ABSTRACT: The strict exchange of protons for sodium ions across cell membranes by Na^+/H⁺ exchangers is a fundamental mechanism for cell homeostasis. At active pH, Na⁺/H⁺ exchange can be modelled as competition between H⁺ and Na⁺ to an ion-binding site, harbouring either one or two aspartic-acid residues. Nevertheless, extensive analysis on the model Na⁺/H⁺ antiporter NhaA from Escherichia coli, has shown that residues on the cytoplasmic surface, termed the pH sensor, shifts the pH at which NhaA becomes active. It was unclear how to incorporate the pH senor model into an alternating-access mechanism based on the NhaA structure at inactive pH 4. Here, we report the crystal structure of NhaA at active pH 6.5, and to

SUBMITTER: Winkelmann I 

PROVIDER: S-EPMC9606361 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature