IGF2BP3 promotes progression of gallbladder carcinoma by stabilizing KLK5 mRNA in N⁶-methyladenosine-dependent binding.

ABSTRACT:

Background

Recent studies have reported that IGF2BP3 is linked to the pathogenesis of various malignancies. Since IGF2BP3 is associated with poor outcomes of gallbladder carcinoma (GBC), we aimed to explore the association between its N⁶-methyladenosine (m6A) RNA methylation and GBC progression.

Methods

Bioinformatic analysis of GSE136982, GSE104165, and RNA-seq was performed. In vitro and in vivo gain- and loss-of-function assays were done. qPCR, Western blotting, and IHC were conducted in cells or in collected clinical tissue samples. RNA immunoprecipitation, RNA stability measurement, methylated RNA immunoprecipitation, and dual-luciferase reporter assays were performed in this study.

Results

The expression of IGF2BP3 was higher in GBC tiss

SUBMITTER: Zhang J 

PROVIDER: S-EPMC9606626 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature