Simple and efficient protocol to isolate and culture brain microvascular endothelial cells from newborn mice.

ABSTRACT: The neurovascular unit (NVU) is a multicellular structure comprising of neurons, glial cells, and non-neural cells, and it is supported by a specialized extracellular matrix, the basal lamina. Astrocytes, brain microvascular endothelial cells (BMECs), pericytes, and smooth muscle cells constitute the blood-brain barrier (BBB). BMECs have a mesodermal origin and invade the nervous system early in neural tube development, forming the BBB anatomical core. BMECs are connected by adherent junction complexes composed of integral membrane and cytoplasmic proteins. In vivo and in vitro studies have shown that, given the proximity and relationship with neural cells, BMECs acquire a unique gene expression profile, proteome, and specific mechanical and physical properties compared to en

SUBMITTER: Nicolicht-Amorim P 

PROVIDER: S-EPMC9606660 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature