Fast Mass Microscopy: Mass Spectrometry Imaging of a Gigapixel Image in 34 Minutes.

ABSTRACT: Mass spectrometry imaging (MSI) maps the spatial distributions of chemicals on surfaces. MSI requires improvements in throughput and spatial resolution, and often one is compromised for the other. In microprobe-mode MSI, improvements in spatial resolution increase the imaging time quadratically, thus limiting the use of high spatial resolution MSI for large areas or sample cohorts and time-sensitive measurements. Here, we bypass this quadratic relationship by combining a Timepix3 detector with a continuously sampling secondary ion mass spectrometry mass microscope. By reconstructing the data into large-field mass images, this new method, fast mass microscopy, enables orders of magnitude higher throughput than conventional MSI albeit yet at lower mass resolution. We acquired submicron, giga

SUBMITTER: Korber A 

PROVIDER: S-EPMC9607864 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature