Differential alternative splicing genes and isoform co-expression networks of Brassica napus under multiple abiotic stresses.

ABSTRACT: Alternative splicing (AS) is an important regulatory process that affects plant development and stress responses by greatly increasing the complexity of transcriptome and proteome. To understand how the AS landscape of B. napus changes in response to abiotic stresses, we investigated 26 RNA-seq libraries, including control and treatments with cold, dehydration, salt, and abscisic acid (ABA) at two different time points, to perform comparative alternative splicing analysis. Apparently, AS events increased under all stresses except dehydration for 1 h, and intron retention was the most common AS mode. In addition, a total of 357 differential alternative splicing (DAS) genes were identified under four abiotic stresses, among which 81 DAS genes existed in at least two stresses, and 276

SUBMITTER: Yang L 

PROVIDER: S-EPMC9608124 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature