A Nanobody-Based Immunoassay for Detection of Ustilaginoidins in Rice Samples.

ABSTRACT: Ustilaginoidins are a class of bis-naphtho-γ-pyrone mycotoxins produced by the pathogen Villosiclava virens of rice false smut, which has recently become one of the most devastating diseases in rice-growing regions worldwide. In this research, the nanobody phage display library was established after an alpaca was immunized with the hemiustilaginoidin F-hapten coupled with bovine serum albumin (BSA). Heterologous antigen selection and combing trypsin with competition alternant elution methods were performed for nanobody screening. Two nanobodies, namely, Nb-B15 and Nb-C21, were selected for the establishment of indirect competitive enzyme-linked immunosorbent assays (ic-ELISAs). For Nb-B15 and Nb-C21, their IC₅₀ values were 11.86 μg/mL and 11.22 μg/mL, and the detection ra

SUBMITTER: Wang W 

PROVIDER: S-EPMC9609001 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature