Engineering of global transcription factor FruR to redirect the carbon flow in Escherichia coli for enhancing L-phenylalanine biosynthesis.

ABSTRACT:

Background

The catabolite repressor/activator protein (FruR) is a global regulatory protein known to control the expression of several genes concerned with carbon utilization and energy metabolism. This study aimed to illustrate effects of the FruR mutant on the _L-phenylalanine (_L-PHE) producing strain PHE01.

Results

Random mutagenesis libraries of fruR generated in vitro were first integrated into the chromosome of PHE01 by CRISPR/Cas9 technique, and then the best mutant PHE07 (FruR^E173K) was obtained. With this mutant, a final _L-PHE concentration of 70.50 ± 1.02 g/L was achieved, which was 23.34% higher than that of PHE01. To better understand the mechanism, both transcriptomes and metabolomes of PHE07 were carried out and compar

SUBMITTER: Chen M 

PROVIDER: S-EPMC9609185 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature