Evaluation of Metabolism of a Defined Pesticide Mixture through Multiple In Vitro Liver Models.

ABSTRACT: The evaluation of exposure to multiple contaminants in a mixture presents a number of challenges. For example, the characterization of chemical metabolism in a mixture setting remains a research area with critical knowledge gaps. Studies of chemical metabolism typically utilize suspension cultures of primary human hepatocytes; however, this model is not suitable for studies of more extended exposures and donor-to-donor variability in a metabolic capacity is unavoidable. To address this issue, we utilized several in vitro models based on human-induced pluripotent stem cell (iPSC)-derived hepatocytes (iHep) to characterize the metabolism of an equimolar (1 or 5 µM) mixture of 20 pesticides. We used iHep suspensions and 2D sandwich cultures, and a microphysiological system OrganoPlate^®</

SUBMITTER: Valdiviezo A 

PROVIDER: S-EPMC9609317 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature