Engineering Cell Polarization Improves Protein Production in Saccharomyces cerevisiae.

ABSTRACT: Saccharomyces cerevisiae has been widely used as a microbial cell factory to produce recombinant proteins. Therefore, enhancing the protein production efficiency of yeast cell factories to expand the market demand for protein products is necessary. Recombinant proteins are often retained in the secretory pathway because of the limited protein transport performed by vesicle trafficking. Cell polarization describes the asymmetric organization of the plasma membrane cytoskeleton and organelles and tightly regulates vesicle trafficking for protein transport. Engineering vesicle trafficking has broadly been studied by the overexpression or deletion of key genes involved but not by modifying cell polarization. Here, we used α-amylase as a reporter protein, and its secretion and surface-di

SUBMITTER: Yang S 

PROVIDER: S-EPMC9609600 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature