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Portuino-A Novel Portable Low-Cost Arduino-Based Photo- and Fluorimeter.

ABSTRACT: A novel portable low-cost Arduino-controlled photo- and fluorimeter for on-site measurements has been developed. The device uses LEDs as a light source and a phototransistor as a light sensor. The circuit is based on the discharge of a capacitor with the photocurrent from the phototransistor. Validation experiments for absorbance measurements were performed by measuring protein concentration using the Bradford method and measuring phosphate ions in water using a commercial test kit. The emission light of the excited fluorescent dyes rhodamine 6G and calcofluor white was measured to validate the usability of the device as a fluorescence photometer. In all validation experiments, similar correlation coefficients and limit of detection could be achieved with the portable photo- and fluorimeter and a laboratory spectrometer and fluorimeter. Real sample analysis was performed, measuring phosphate concentration in freshwater and concentration of green fluorescent protein, extracted from Escherichia coli.

SUBMITTER: Di Nonno S

PROVIDER: S-EPMC9609715 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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Portuino-A Novel Portable Low-Cost Arduino-Based Photo- and Fluorimeter.

Di Nonno Sarah S Ulber Roland R

Sensors (Basel, Switzerland) 20221018 20

A novel portable low-cost Arduino-controlled photo- and fluorimeter for on-site measurements has been developed. The device uses LEDs as a light source and a phototransistor as a light sensor. The circuit is based on the discharge of a capacitor with the photocurrent from the phototransistor. Validation experiments for absorbance measurements were performed by measuring protein concentration using the Bradford method and measuring phosphate ions in water using a commercial test kit. The emission ...[more]

PMID: 36298268

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