Mirubactin C rescues the lethal effect of cell wall biosynthesis mutations in <i>Bacillus subtilis</i>.

ABSTRACT: Growth of most rod-shaped bacteria is accompanied by the insertion of new peptidoglycan into the cylindrical cell wall. This insertion, which helps maintain and determine the shape of the cell, is guided by a protein machine called the rod complex or elongasome. Although most of the proteins in this complex are essential under normal growth conditions, cell viability can be rescued, for reasons that are not understood, by the presence of a high (mM) Mg²⁺ concentration. We screened for natural product compounds that could rescue the growth of mutants affected in rod-complex function. By screening > 2,000 extracts from a diverse collection of actinobacteria, we identified a compound, mirubactin C, related to the known iron siderophore mirubactin A, which rescued growth in the low

SUBMITTER: Kepplinger B 

PROVIDER: S-EPMC9609785 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature