The Application of MALDI-TOF MS for a Variability Study of Paenibacillus larvae.

ABSTRACT: In recent decades, the significant deterioration of the health status of honey bees has been observed throughout the world. One of the most severe factors affecting the health of bee colonies worldwide is American foulbrood disease. This devastating disease, with no known cure, is caused by the Gram-positive spore-forming bacteria of Paenibacillus larvae species. At present, DNA-based methods are being used for P. larvae identification and typing. In our study, we compare two of the most advanced DNA-based technologies (rep-PCR and 16S rRNA analyses) with MALDI-TOF MS fingerprinting to evaluate P. larvae variability in Central Europe. While 16S rRNA analysis presents a very limited variation among the strains, MALDI-TOF MS is observed to be more efficient at differenti

SUBMITTER: Kopcakova A 

PROVIDER: S-EPMC9610059 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature