Acetyl Co-A Carboxylase Inhibition Halts Hyperglycemia Induced Upregulation of De Novo Lipogenesis in Podocytes and Proximal Tubular Cells.

ABSTRACT: The effect of glycemic stress on de novo lipogenesis (DNL) in podocytes and tubular epithelial cells is understudied. This study is aimed (A) to show the effect of glycemic stress on DNL, and (B) to assess the effect of acetyl-Co A (ACC) inhibition on halting upregulation of DNL, on the expression of other lipid regulatory genes in the DNL pathway, and on markers of fibrosis and apoptosis in podocytes and tubular epithelial cells. We used cultured mouse primary tubular epithelial cells, mouse proximal tubular (BUMPT) cells, and immortal mouse podocytes and measured their percentage of labeled ¹³C₂-palmitate as a marker of DNL after incubation with ¹³C₂ acetate in response to high glucose concentration (25 mM). We then tested the effect of ACC inh

SUBMITTER: Kayampilly P 

PROVIDER: S-EPMC9610518 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature