Consumption of Galactose by Trypanosoma cruzi Epimastigotes Generates Resistance against Oxidative Stress.

ABSTRACT: In this study, we demonstrate that Trypanosoma cruzi epimastigotes previously grown in LIT medium supplemented with 20 mM galactose and exposed to sub-lethal concentrations of hydrogen peroxide (100 μM) showed two-fold and five-fold viability when compared to epimastigotes grown in LIT medium supplemented with two different glucose concentrations (20 mM and 1.5 mM), respectively. Similar results were obtained when exposing epimastigotes from all treatments to methylene blue 30 μM. Additionally, through differential centrifugation and the selective permeabilization of cellular membranes with digitonin, we found that phosphoglucomutase activity (a key enzyme in galactose metabolism) occurs predominantly within the cytosolic compartment. Furthermore, after partially permeabilizing epim

SUBMITTER: Lobo-Rojas A 

PROVIDER: S-EPMC9611177 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature