Highly selective fluorescent probe with an ideal pH profile for the rapid and unambiguous determination of subcellular labile iron (III) pools in human cells.

ABSTRACT: A convenient tool for detecting iron ions in subcellular structures is desired for better understanding its roles in biological systems. In this work, a new Fe³⁺ sensor, 2-(2-((1-(6-acetylpyridin-2-yl)ethylidene)amino)ethyl)-3',6'-bis(diethylamino)spiro[isoindoline-1,9'-xanthen]-3-one (RhPK), which operates across the entire cellular pH range and is capable of unambiguously detecting Fe³⁺ ion in live human cells at subcellular resolution, is reported. The sensor exhibits high selectivity and sensitivity toward Fe³⁺ with a rapid fluorescence response and a 12-fold increase in intensity upon the addition of 1 equivalent Fe³⁺ at pH 7.3. RhPK forms a 1:1 complex with Fe³⁺ with an apparent binding constant 1.54 × 10⁷ M^-1

SUBMITTER: Alhawsah B 

PROVIDER: S-EPMC9611270 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature