Dataset Information

Germination Behavior and Early Seedling Growth in Abies pinsapo Boiss. Seeds.

ABSTRACT: Abies pinsapo Boiss. is a conifer endemic to southern Spain. It is categorized as an endangered species in the IUCN list and the plant communities it forms are considered unique ecosystems, being the remains of fir forests occupying the Mediterranean basin during the last glaciations. Understanding seed germination and plant production is essential for the management and conservation of A. pinsapo stands. The objective of this work was to investigate the effect of temperature and light on germination behavior and early seedling growth of A. pinsapo seeds from different populations. The results obtained reveal a significant influence of seed origin on germination percentage. Temperature played a critical role on germination rate, with optimal results at 15 °C. Light only significantly affected germination in seeds from Grazalema, although significant population × light and temperature x light interactions could be inferred. In relation to germination kinetics, different responses to the environmental factors tested were observed among seeds from different provenances. Globally, the temperature influenced all the germination parameters, except germination onset. However, light affected germination initiation and speed. Early seedling growth depended on seed origin and temperature. The temperature played a determinant role as temperatures above 15 °C strongly limited plantlets development. Light only significantly influenced root length in plantlets obtained from Grazalema seeds.

SUBMITTER: Bravo-Navas MV

PROVIDER: S-EPMC9611577 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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Germination Behavior and Early Seedling Growth in Abies pinsapo Boiss. Seeds.

Bravo-Navas María Victoria MV Sánchez-Romero Carolina C

Plants (Basel, Switzerland) 20221014 20

Abies pinsapo Boiss. is a conifer endemic to southern Spain. It is categorized as an endangered species in the IUCN list and the plant communities it forms are considered unique ecosystems, being the remains of fir forests occupying the Mediterranean basin during the last glaciations. Understanding seed germination and plant production is essential for the management and conservation of A. pinsapo stands. The objective of this work was to investigate the effect of temperature and l ...[more]

PMID: 36297738

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Project description:Abies pinsapo is an endangered species, endemic to southern Spain. Somatic embryogenesis and cryopreservation constitute important biotechnological tools, which can be used in order to improve the management and conservation of threatened species. The objective of this work was to develop somatic embryogenesis and cryopreservation protocols for A. pinsapo. Somatic embryogenesis was induced from mature zygotic embryos of A. pinsapo cultured on solid MS medium with macroelements at half-strength and supplemented with 20 g L-1 sucrose and 5 mg L-1 6-benzylaminopurine (BA). Embryogenic cultures successfully proliferated on solid medium with the same formulation supplemented with 20 g L-1 sucrose, 500 mg L-1 L-glutamine, 1 g L-1 casein hydrolysate and 1 mg L-1 BA. Different preconditioning and cryoprotective treatments were tested in order to optimize cryopreservation of embryogenic tissues by using the slow-cooling technique. Embryogenic cultures at their exponential growth phase, i.e. 12-14 days after the last subculture, were used as cryopreservation explants. The best results were achieved after sucrose preculture and cryoprotecion with PGD I (mixture of polyethylene glycol, glucose and DMSO I), with 100% of explants resuming somatic embryogenesis after thawing. Following fluorescein diacetate (FDA) staining, more intense and abundant green fluorescence could be observed in these samples, compared to those subjected to other preconditioning and cryoprotective treatments, thus evincing a higher proportion of viable cells after freezing in liquid nitrogen. Cold hardening did not improve cryotolerance. In fact, incubation at 5 °C for two weeks appeared to affect explants response, delaying tissue regrowth after cryopreservation. This is the first time in which somatic embryogenesis and cryopreservation have been reported in Spanish fir. The results obtained allow to establish the bases for the integration of these techniques into in situ and ex situ conservation strategies.

Dataset Information

Germination Behavior and Early Seedling Growth in Abies pinsapo Boiss. Seeds.

Publications

Germination Behavior and Early Seedling Growth in <i>Abies pinsapo</i> Boiss. Seeds.

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