Dataset Information

Proteomic Study of Response to Copper, Cadmium, and Chrome Ion Stress in Yarrowia lipolytica Strains Isolated from Andean Mine Tailings in Peru.

ABSTRACT: Mine tailings are produced by mining activities and contain diverse heavy metal ions, which cause environmental problems and have negative impacts on ecosystems. Different microorganisms, including yeasts, play important roles in the absorption and/or adsorption of these heavy metal ions. This work aimed to analyze proteins synthesized by the yeast Yarrowia lipolytica AMJ6 (Yl-AMJ6), isolated from Andean mine tailings in Peru and subjected to stress conditions with common heavy metal ions. Yeast strains were isolated from high Andean water samples impacted by mine tailings from Yanamate (Pasco, Peru). Among all the isolated yeasts, the Yl-AMJ6 strain presented LC50 values of 1.06 mM, 1.42 mM, and 0.49 mM for the Cr⁺⁶, Cu⁺², and Cd⁺² ions, respectively. Proteomic analysis of theYl-AMJ6 strain under heavy metal stress showed that several proteins were up- or downregulated. Biological and functional analysis of these proteins showed that they were involved in the metabolism of proteins, nucleic acids, and carbohydrates; response to oxidative stress and protein folding; ATP synthesis and ion transport; membrane and cell wall; and cell division. The most prominent proteins that presented the greatest changes were related to the oxidative stress response and carbohydrate metabolism, suggesting the existence of a defense mechanism in these yeasts to resist the impact of environmental contamination by heavy metal ions.

SUBMITTER: Sanchez-Rojas T

PROVIDER: S-EPMC9611812 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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Proteomic Study of Response to Copper, Cadmium, and Chrome Ion Stress in <i>Yarrowia lipolytica</i> Strains Isolated from Andean Mine Tailings in Peru.

Sánchez-Rojas Tito T Espinoza-Culupú Abraham A Ramírez Pablo P Iwai Leo Kei LK Montoni Fabio F Macedo-Prada Diego D Sulca-López Marcos M Durán Yerson Y Farfán-López Mariella M Herencia Jennifer J

Microorganisms 20221011 10

Mine tailings are produced by mining activities and contain diverse heavy metal ions, which cause environmental problems and have negative impacts on ecosystems. Different microorganisms, including yeasts, play important roles in the absorption and/or adsorption of these heavy metal ions. This work aimed to analyze proteins synthesized by the yeast <i>Yarrowia lipolytica</i> AMJ6 (Yl-AMJ6), isolated from Andean mine tailings in Peru and subjected to stress conditions with common heavy metal ions ...[more]

PMID: 36296278

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Project description:Copper mining, the central axis of Chile's economic development, produces a large number of tailings, which become a potential environmental risk. This study aims to evaluate the mechanical properties resulting from the making of Portland cement mixtures with tailings as aggregates so that they can be eventually used in paving stones for building inactive tailings dams. Tailings coming from two dams at a concentration plant located in Taltal (Chile) were used. Currently, Dam 1 is inactive, while Dam 2 is active. The tailings samples obtained from both dams were granulometrically characterized by sieving. In addition, pH, humidity, Eh, and mineralogical assays (sulfides, oxides, sulfates, carbonates, phosphates, and silicates) were measured. The fines content of the tailings from Dams 1 and 2 with a sieve size of N°200 ASTM were 76.2% and 29.6%, respectively. Therefore, owing to their high percentage of fines, they cannot be as used as concrete aggregates. Aggregates must contain a maximum percentage of fines so that mortars and concrete can meet Chilean standards. In this paper, to comply with a 7% and 15% fines content lower than 0.075 mm, tailings materials were mixed with conventional aggregates containing very little fines. In addition, a reference mixture was made with only tailings aggregates with and without a superplasticizer additive. To measure the mixtures of cement, aggregates, and tailings, bending and compression strength assays were made of the specimens after a 28-day curing, according to the Chilean standard. The results of the study show that the addition of only part of the tailings to the mixture increases bending strength by 26% and compression strength by 180% compared with the reference mortar, with a fines content lower than 0.075 mm in the 7% mixture, thus allowing paving stone manufacture with tailings materials. In addition, it was possible to increase the workability of the reference mixture by using superplasticizers as additives.

Project description:The oxidation of sulfide-bearing mine tailings catalyzed by acidophilic iron and sulfur-oxidizing bacteria releases toxic metals and other contaminants into soil and groundwater as acid mine drainage. Understanding the environmental variables that control the community structure and metabolic activity of microbes indigenous to tailings (especially the abiotic stressors of low pH and high dissolved metal content) is crucial to developing sustainable bioremediation strategies. We determined the microbial community composition along two continuous vertical gradients of Cu/Ni mine tailings at each of two tailings impoundments near Sudbury, Ontario. 16S rRNA amplicon data showed high variability in community diversity and composition between locations, as well as at different depths within each location. A temporal comparison for one tailings location showed low fluctuation in microbial communities across 2 years. Differences in community composition correlated most strongly with pore-water pH, Eh, alkalinity, salinity, and the concentration of several dissolved metals (including iron, but not copper or nickel). The relative abundances of individual genera differed in their degrees of correlation with geochemical factors. Several abundant lineages present at these locations have not previously been associated with mine tailings environments, including novel species predicted to be involved in iron and sulfur cycling.IMPORTANCEMine tailings represent a significant threat to North American freshwater, with legacy tailings areas generating acid mine drainage (AMD) that contaminates rivers, lakes, and aquifers. Microbial activity accelerates AMD formation through oxidative metabolic processes but may also ameliorate acidic tailings by promoting secondary mineral precipitation and immobilizing dissolved metals. Tailings exhibit high geochemical variation within and between mine sites and may harbor many novel extremophiles adapted to high concentrations of toxic metals. Characterizing the unique microbiomes associated with tailing environments is key to identifying consortia that may be used as the foundation for innovative mine-waste bioremediation strategies. We provide an in-depth analysis of microbial diversity at four copper/nickel mine tailings impoundments, describe how communities (and individual lineages) differ based on geochemical gradients, predict organisms involved in AMD transformations, and identify taxonomically novel groups present that have not previously been observed in mine tailings.

Dataset Information

Proteomic Study of Response to Copper, Cadmium, and Chrome Ion Stress in Yarrowia lipolytica Strains Isolated from Andean Mine Tailings in Peru.

Publications

Proteomic Study of Response to Copper, Cadmium, and Chrome Ion Stress in <i>Yarrowia lipolytica</i> Strains Isolated from Andean Mine Tailings in Peru.

Similar Datasets

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